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Last Updated: 03/08/2023

AKT Multiplex Immunoassay (Panels 1-3)
ERK/MEK Multiplex Immunoassay (Panels 4-5)

The Signaling Multiplex Immunoassays are novel isoform specific signaling multiplex immunoassay panels of 12 biomarkers for AKT and rpS6 and 8 biomarkers for MEK and ERK to measure on target or downstream pathway modulation by multiple classes of drugs including PI3K K/AKT/ mTORC and RAS/RAF/MEK/ERK inhibitors The AKT/rpS6 multiplex immunoassays were built on the Luminex platform by grouping 12 biomarkers into three panels Signaling Multiplex Panel 1 ("Total AKT/rpS6 Panel") detects total levels of AKT1, AKT2, AKT3, and rpS6 Signaling Multiplex Panel 2 ("Phospho AKT/rpS6 A Panel") detects pSer473-AKT1, pSer474-AKT2, pSer472-AKT3, and pSer235-rpS6 and Signaling Multiplex Panel 3 ("Phospho AKT/rpS6 B Panel") detects pThr308-AKT1, pThr309-AKT2, pThr305-AKT3, and pSer240/244-rpS6. The MEK/ERK multiplex immunoassays were built on the Luminex platform by grouping 8 biomarkers into two panels Signaling Multiplex Panel 4 ("Total MEK/ERK Panel") measures total levels of ERK1, ERK2, MEK1, and MEK2; Signaling Multiplex Panel 5 ("Phospho MEK/ERK Panel") measures pThr202/Y204-ERK1, pThr185/Y187-ERK2, pSer218/222-MEK1, and pSer222/226-MEK2. Total lysates from a single core needle biopsy is sufficient to evaluate all five multiplexes.

  1. Specimen Collection
  2. Sample Preparation
  3. Kit Use and Data Reporting SOP

Please email any questions or comments to PDinfo@mail.nih.gov.