Apoptosis Multiplex Immunoassay

The Apoptosis Multiplex Immunoassay is a novel multiplex immunoassay panel of 13 biomarkers indicative of the induction, onset, and commitment to apoptosis. The multiplex immunoassays were built on the Bio-Plex platform by grouping 13 biomarkers into three panels. Panel 1 contains BAK, BAX, Lamin B (intact and 45 kDa fragment), and SMAC; Panel 2 contains BAD, BAX:BCL2 heterodimer, BCL-XL, BIM, and MCL1; and Panel 3 contains active caspase 3, BCL-XL:BAK heterodimer, MCL1:BAK heterodimer, and Survivin.

1. Specimen Collection
   • Shipment to PADIS, FNLCR
   • Shipment to EET Biobank
2. Sample Preparation
3. Kit Use and Data Reporting SOP

• Assay Training and Key Reagent Support
• NCI Publications