Assays Available through the NCI National Clinical Laboratory Network (NCLN)

Assay	Tissue	Specimen Collection	Sample Preparation	Assay(s)	Data Analysis
Apoptosis Multiplex, Luminex	tumor	SOP340507 Tumor Frozen Needle Biopsy Specimen Collection and Handling	SOP341401 Tumor Biopsy Lysate Fractionation for the Apoptosis Multiplex Immunoassay Panels	SOP341402 Procedure for Use of Bio-Rad Bio-Plex Pro RBM Apoptosis Panel Kits 1-3 for the Analysis of Clinical Tumor Biopsy Extracts and Reporting of Data
γH2AX, pNBS1, and β-catenin Multiplex, IFA	tumor	SOP340507 Tumor Frozen Needle Biopsy Specimen Collection and Handling	SOP340550 Tumor Frozen Needle Biopsy Preparation for Pharmacodynamic Immunofluorescence Assays Utilizing Murine Testis and/or Jejunum Control Tissues	SOP340543, γH2AX, pNBS1 IFA Staining with β-Catenin Segmentation for Tumor Biopsy Slides SOP340544, Whole Slide Image Capture of Tumor Biopsy Slides for γH2AX, pNBS1 Immunofluorescence Assay with β-Catenin Segmentation	SOP340545 Image Extraction and Analysis of Tumor Biopsy Slides from γH2AX, pNBS1 IFA with β-Catenin Segmentation

Assay

Tissue

Specimen Collection

Sample Preparation

Assay(s)

Data Analysis

Apoptosis Multiplex, Luminex

tumor

SOP340507
Tumor Frozen Needle Biopsy Specimen Collection and Handling

SOP341401
Tumor Biopsy Lysate Fractionation for the Apoptosis Multiplex Immunoassay Panels

SOP341402
Procedure for Use of Bio-Rad Bio-Plex Pro RBM Apoptosis Panel Kits 1-3 for the Analysis of Clinical Tumor Biopsy Extracts and Reporting of Data

γH2AX, pNBS1, and β-catenin Multiplex, IFA

tumor

SOP340507
Tumor Frozen Needle Biopsy Specimen Collection and Handling

SOP340550
Tumor Frozen Needle Biopsy Preparation for Pharmacodynamic Immunofluorescence Assays Utilizing Murine Testis and/or Jejunum Control Tissues

SOP340543, γH2AX, pNBS1 IFA Staining with β-Catenin Segmentation for Tumor Biopsy Slides

SOP340544, Whole Slide Image Capture of Tumor Biopsy Slides for γH2AX, pNBS1 Immunofluorescence Assay with β-Catenin Segmentation

SOP340545
Image Extraction and Analysis of Tumor Biopsy Slides from γH2AX, pNBS1 IFA with β-Catenin Segmentation