About the Nanotechnology Characterization Lab (NCL)
NCL Overview
Services Provided by the Nanotechnology Characterization Lab
The Nanotechnology Characterization Lab is a fully equipped, multidisciplinary resource for investigators developing nano-based therapies. Learn more about the services available through collaboration with the NCL.
NCI founded the Nanotechnology Characterization Lab (NCL) in 2004, in collaboration with the U.S. Food and Drug Administration and National Institute of Standards and Technology, as a public–private partnership. It was founded to advance the science needed to expedite the development of promising nanotech therapies and diagnostics. The NCL’s initial mission was to develop an “Assay Cascade” of scientific tests that would help determine the reproducibility, safety, and efficacy of nanomedicines to facilitate regulatory review. The NCL is the only lab with experience testing the wide variety of platforms used in nanomedicine. Since its founding, the NCL has tested more than 500 unique nanomaterials, including almost every type of nanoparticle used in biomedical research and development (e.g., metallic, liposomes, polymers, proteins, micelles, DNA and RNA nanostructures, carbon nanotubes) with nearly every type of active pharmaceutical ingredient (e.g., small molecules, peptides, proteins, nucleic acids, plasmids).
The NCL assists the extramural nanotechnology research community by providing characterization and formulation assistance. The goal of these efforts is to assist developers with preclinical development and clinical translation of their products. Characterization includes comprehensive physicochemical analysis, as well as in vitro and in vivo analysis of immunology, pharmacology, and toxicology properties. Nanotech characterization services are available through the free Assay Cascade program and the cCRADA mechanism, and for select assays, the technical services mechanism. Nanotech reformulation services are available through the cCRADA mechanism.
The NCL is located at the Advanced Technology Research Facility in Frederick, Maryland. It has over 11,000 square feet of laboratory and office space. The laboratories are outfitted with two tissue culture rooms, an isotope laboratory, a walk-in cold room, several microscopy rooms, a chromatography and electrophoresis area, a spectroscopy area, a formulation/synthetic lab with chemical fume hoods, multiple customizable research bays, and open floor space for freestanding instrumentation and equipment. The NCL also has direct access to all resources at the Frederick National Laboratory for Cancer Research, including those co-located with the NCL at the Advanced Technology Research Facility.
NCL Annual Report
2023 NCL Annual Report
NCL's annual publication of metrics on NCL's Assay Cascade characterization program, including application and submission trends. Highlighted are a new application mechanism intended to reach an even broader audience, new protocols added to the NCL's Assay Cascade, and many non-characterization support opportunities. The report also features success stories from several recent collaborators.
Contact the NCL
Nanotechnology Characterization Laboratory
8560 Progress Drive
Frederick, MD 21701
Phone: 301-846-6939
Fax: 301-846-6399
Email: ncl@mail.nih.gov
NCL Staff
Marina A. Dobrovolskaia, Ph.D., M.B.A., P.M.P.
Dr. Marina Dobrovolskaia serves as the NCL Director of Operations and manages the immunology section.
301-228-4935 | marina@mail.nih.gov
Stephan T. Stern, Ph.D., D.A.B.T.
Dr. Stephan Stern serves as the NCL Director of Research and Development and Head of the pharmacology and toxicology section as well as the formulation team.
240-549-2553 | sternstephan@mail.nih.gov
Jeffrey D. Clogston, Ph.D.
Dr. Jeffrey Clogston is a Principal Scientist at the NCL. He manages the physicochemical characterization section.
301-846-1388 | clogstonj@mail.nih.gov
Rachael M. Crist, Ph.D.
Dr. Rachael Crist is a Scientist and Head of the NCL's Strategic Client Relations. She manages the lab's extramural collaborations, serves as the primary point of contact for collaborators, and participates on the NCL's Assay Cascade review committee.
NCL Publications
View List of NCL’s Most Recent Publications <UPDATE LINK>
NCL Collaborators
All of NCL's programs (Assay Cascade, cCRADA, Technical Services) are open to researchers from any institution. Non-U.S. organizations are also eligible.