Requesting Materials

Data are generated from PDX tumors, which are a mixture of human tumor and mouse stroma. These are not pure human extractions. Murine reads are removed bioinformatically. Find the data analysis pipelines and reference sequence for the NSG host (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) on the PDMR SOP page.

Details and pricing can be found in the Request Procedures under Forms and Associated Documents below.

Detailed information about PDMR models including genomic and transcriptomic analysis can be accessed in the NCI Patient Derived Models Repository database (PDMR database).

1. Fill out a PDMR Model Request Form.
   • Provide no more than a 2-page summary of the research plan for the requested material(s).
   • Intramural investigators (MD campuses – Bethesda, Shady Grove, Frederick) – leave the FedEx and Payment Information blank

2. Review the Material Transfer Terms of Agreement and fill out the Material Transfer Agreement (MTA) with all requested materials included in the appropriate Appendix as outlined below.  Ensure the requesting site’s authorizing official signature is affixed. Modifications to NCI’s Material Transfer Terms of Agreement will not be considered.

         Appendix 1: PDX model Distribution Lot names listed and material type requested.

   • If requesting cryopreserved fragments for PDX generation, provide a copy of your Animal Care and Use Committee (ACUC) protocol. You must indicate your laboratory or designate uses NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice or a Bill of Sale showing NSG mice have been purchased. All NCI Patient Derived Models Repository PDXs must be initially implanted into NSG mice by the recipient for propagation.
   • If the recipient wishes to expand in other host mouse strains, the recipient should establish a stock of viably cryopreserved fragments from the initial NSG implants and then test PDX take-rates in alternate host strains.

         Appendix 2: PDC, CAF, and/or PDOrg model Distribution Lot names requested.

   • If requesting in vitro material, a follow up email will be sent after request documents have been received indicating the specific defined media requirements of the models requested. The recipient must acknowledge that they are aware and will purchase the media components before their request is sent for review.
   • If requesting CAF cultures, be sure to supply sufficient justification for why the proposed research should be considered over other requestors. CAFs are non-renewable by the PDMR and once distribution material is used up, no further material will be available.

         Appendix 3: Tissue Microarray (TMA) Panel(s) name, the number of slides being requested, and if you want consecutive/non-consecutive slides. List of currently available PDX TMAs listed below. New histology-focused panels will be added periodically.

   • List of currently available PDX TMAs listed below.  New histology-focused panels will be added periodically.
   • If requesting TMA slide(s), include confirmation that antibody optimization will be performed on control tissues prior to use on the TMA.
   • Note a maximum of 3 slides per TMA panel can be requested by an individual investigator. Specific slide numbers may not be requested.
   • Non-consecutive slides will be a minimum of 15 slides apart. As blocks are depleted, non-consecutive slide requests may not fit these criteria, and the Requestor will be contacted.
3. Send the (a) Request Form, (b) signed MTA, and (c) ACUC protocol or prior Bill of Sale and copy of purchase order (if applicable) to: NCI_PDM_Repository@mail.nih.gov

Cryopreserved and Flash Frozen Material

Tissue Microarrays

Previous Material Transfer Terms of Agreement

Material Transfer Terms of Agreement ver. 2025-05-01
Material Transfer Terms of Agreement ver. 2021-09-01

Associated Documents

NCI PDMR Annual Report
NCI PDMR Material Request Procedures
NCI PDMR Material Information

1. The PDMR can accept payments by (i) electronic ACH/Wire transfer or (ii) mailed paper checks (see payment procedures in PDMR Material Request Procedures document).
2. Upon receipt of viably cryopreserved material, vial(s) should be immediately placed in LN2 Vapor Phase (-180°C); Do not store at -80°C.
3. All NCI Patient-Derived Models Repository (PDMR) in vitro cultures (PDOrg, PDC, and CAF) are tested for sterility and confirmed mycoplasma free.

For questions or comments, please e-mail us at NCI_PDM_Repository@mail.nih.gov