γH2AX, pNBS1, and β-Catenin Multiplex Immunofluorescence Assay

The γH2AX, pNBS1 IFA with β-Catenin segmentation is an immunofluorescence-based staining assay developed to quantify γH2AX and pNBS1 using β-Catenin staining to segment tumor from non-tumor to precisely limit quantitation of these DNA damage response markers to the tumor cells within a tumor biopsy section.

• Specimen Collection
  • Shipment to PADIS, FNLCR
  • Shipment to EET Biobank
• Sample Preparation
• Assay SOP
• Image Capture
• Image and Data Analysis
• NCI Publications
• Assay Training and Key Reagent Support