NCI BEBPs are fit-for-purpose procedural guidelines based on published data and expert recommendations. The detailed, yet adaptable, format was designed to help individual projects, biobanks, and organizations create evidence-based standard operating procedures (SOPs).
Each BEBP includes:
- step-by-step procedural guidelines,
- optimal procedures and acceptable alternatives,
- topic-based summaries of literature evidence,
- a hyperlinked bibliography, and
- recommendations of an expert panel.
BEBP development is ongoing, with new content added as it becomes available.
Available BEBPs
The NCI BEBP on Cell-free miRNA (cfmiRNA): Blood Collection and Processing is customized for cfmiRNA analysis of plasma and serum in research and clinical settings.
The NCI BEBP: Cell-free DNA (cfDNA) Biospecimen Collection and Processing is customized for fetal or tumor cfDNA analysis of plasma in research and clinical settings.
The NCI BEBP on Nucleic Acid Extraction from Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue is customized for DNA and RNA analysis of FFPE soft tissues by PCR, methylation analysis, Sanger sequencing, next-generation sequencing, and microarray hybridization.
The NCI BEBP on Snap-Freezing Post-Surgical Tissue Biospecimens is customized for DNA, RNA, protein, and morphological analysis of surgically collected tissue.